England's backup goalkeeper Hannah Hampton has left the team camp after testing positive for COVID-19, the Women's European Championship hosts said on Tuesday.
Hampton, who made her senior England debut earlier this year, has not yet featured at the Euros, where the Lionesses qualified for the knockout stages as Group A winners.
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"Hannah Hampton has tested positive for COVID. She will be monitored regularly with a view to returning to the squad as soon as possible," England said in a statement.
The 21-year-old is the second England player to contract the virus, after defender Lotte Wubben-Moy returned a positive test last week.
Head coach Sarina Wiegman missed England's win over Northern Ireland on Friday after she tested positive.
England will face Spain in Wednesday's quarterfinal at the Amex Stadium in Brighton.
Meanwhile, Wiegman hopes to be back on the touchline for Wednesday's clash against Spain.
She was forced to relay information via a phone line during the 5-0 hammering of Northern Ireland, but she has been on the training pitch, albeit from a safe distance.
"I'm very hopeful. But we'll see what happens. And we do know if I can't be there. I'll be around in another way," Wiegman said at a virtual news conference on Tuesday.
"In the last game we had a line. So I was in contact with the technical staff all the time during the game so we stay connected all the time.
"If necessary, that's what we do tomorrow again."
Wiegman said the threat of positive COVID-19 tests is ever-present but is relaxed about it, saying extra precautions were being taken.
"We're really aware. We're very careful. It's very invisible. But we try to stay in our bubble and do the right things and, hopefully, we keep everyone fit and when there is a positive then we have to be creative and continue with the ones who we have," Wiegman said.
Spain began the tournament as favourites but hosts England have now taken that role after scoring 14 unanswered goals in the group phase, including an 8-0 rout of Norway.
Spain are without Ballon D'Or winner Alexia Putellas who was ruled out of the tournament with an ACL injury and are also missing experienced forward Jennifer Hermoso but Wiegman knows they still pose a huge obstacle with their possession game.
"Of course they are missing two key players but I still think they have a very good team, and they're very tight on the ball. Absolutely it's going to be a top game tomorrow," she added.
"They'll probably have the ball a lot, but I hope we'll have it too. I do think we're comfortable on the ball so I'm excited about what it will look like tomorrow."