Italy have announced midfielder Claudio Marchisio has withdrawn from their squad for the matches against Liechtenstein and Germany as a precaution.
Marchisio was back in the squad after missing Euro 2016 having ruptured anterior cruciate ligaments while playing for Juventus in April.
The 30-year-old, who has 54 caps, made his return to action last month in his club's 4-1 Serie A win against Sampdoria and has featured twice more since then.
A statement on the Italian Football Federation's official website on Monday said Marchisio had "a left lower limb joint overload" and that boss Giampiero Ventura "as a precaution decided to give up" the player.
The statement added that Atalanta midfielder Roberto Gagliardini and Genoa defender Armando Izzo have been called up.
#Nazionale#Marchisio lascia il ritiro. Il Ct #Ventura convoca #Izzo e #Gagliardini: https://t.co/lC2XGR0tIl#VivoAzzurro #Azzurri #Italia pic.twitter.com/P72A3qkPwl
- Nazionale Italiana (@Vivo_Azzurro) November 7, 2016
Also on Monday, it was announced Fiorentina's Davide Astori had come into the squad as a replacement for fellow defender Andrea Barzagli, who suffered a dislocated shoulder early in Juventus' 2-1 win at Chievo on Sunday.
Italy play Liechtenstein away in a World Cup qualifier on Saturday, then host Germany in a friendly three days later.