Barcelona club staff announced a work stoppage on Monday, a day after a crackdown by Spanish police trying to block referendum voting in Catalonia turned violent.
After calls for Barcelona's La Liga game against Las Palmas on Sunday to be postponed because of the political climate, it went ahead but was played behind closed doors at the Camp Nou.
The club are off for the international break this week, but said via statement on Monday that none of the teams would train as part of the "standstill."
"FC Barcelona joins the country wide strike called for by Table for Democracy and therefore the Club will be closed tomorrow," the club said.
"None of the professional teams or the youth teams at FC Barcelona will train tomorrow at the Ciutat Esportiva."
FC Barcelona joins the country wide strike called for by Table for Democracy and therefore the Club will be closed tomorrow.
— FC Barcelona (@FCBarcelona) October 2, 2017
Barcelona do not play again until travelling to face Atletico Madrid on Oct. 14, after the international break.
Club officials held an emergency meeting on Monday and were expected to release a statement later in the day.
Fellow Catalan club Girona also suspended practice on Tuesday, while Espanyol will have their players undergo physical activities behind closed doors.
After Sunday's game, an emotional Gerard Pique said there were "pros and cons" for playing, and amid the uncertainty said it was possible that he could quit playing for Spain before next summer's World Cup.
''Han actuado con profesionalidad y de modo proporcional y proporcionado''. https://t.co/FLHvhmwJ8L
— Gerard Piqué (@3gerardpique) October 2, 2017
On Monday, Pique posted a video of a clash between Spanish police and protesters, saying about the police: "They acted with professionalism and with a fair and even manner."
Catalan officials say an overwhelming majority of voters supported independence from Spain, but the central government in Madrid has repeatedly condemned the referendum as illegal, unconstitutional and invalid.